A novel speaker segmentation approach based on deep neural network is proposed and investigated. This approach uses deep speaker vectors (d-vectors) to represent speaker characteristics and to find speaker change points. The d-vector is a kind of frame-level speaker recognition feature, whose discriminative training process corresponds to the goal of discriminating a speaker change point from a single speaker speech segment in a short time window. Following the traditional metric-based segmentation, each analysis window contains two sub-windows and is shifting along the audio stream to detect speaker change points, where the speaker characteristics are represented by the means of deep speaker vectors for all frames in each window. Experimental investigations conduced in fast speaker change scenarios show that the proposed method can detect speaker change points more quickly and more effectively than the commonly used segmentation methods.