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THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA

Abstract: 

We propose an automatic 3D segmentation algorithm for multiphoton microscopy images of microglia. Our method is capable of segmenting tubular and blob-like structures from noisy images. Current segmentation techniques and software fail to capture the fine processes and soma of the microglia cells, useful for the study of the microglia role in the brain during healthy and diseased states. Our coupled tubularity flow field (TuFF)-blob flow field (BFF) method evolves a level set towards the object boundary using the directional tubularity and blobness measure of 3D images. Our method found a 20% performance increase against state of the art segmentation methods on a dataset of 3D images of microglia even in images with intensity heterogeneity throughout the object. The coupled TuFF-BFF segmentation results also yielded 40% improvement in accuracy for the ramification index of the processes, which displays the efficacy of our method.

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Paper Details

Authors:
Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton
Submitted On:
7 October 2018 - 11:09am
Short Link:
Type:
Presentation Slides
Event:
Presenter's Name:
Tiffany Ly
Paper Code:
ICIP18001
Document Year:
2018
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Document Files

icip18.pdf

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[1] Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton, "THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA", IEEE SigPort, 2018. [Online]. Available: http://sigport.org/3592. Accessed: May. 21, 2019.
@article{3592-18,
url = {http://sigport.org/3592},
author = {Tiffany Ly; Jeremy Thompson; Tajie Harris; and Scott T. Acton },
publisher = {IEEE SigPort},
title = {THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA},
year = {2018} }
TY - EJOUR
T1 - THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA
AU - Tiffany Ly; Jeremy Thompson; Tajie Harris; and Scott T. Acton
PY - 2018
PB - IEEE SigPort
UR - http://sigport.org/3592
ER -
Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton. (2018). THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA. IEEE SigPort. http://sigport.org/3592
Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton, 2018. THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA. Available at: http://sigport.org/3592.
Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton. (2018). "THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA." Web.
1. Tiffany Ly, Jeremy Thompson, Tajie Harris, and Scott T. Acton. THE COUPLED TUFF-BFF ALGORITHM FOR AUTOMATIC 3D SEGMENTATION OF MICROGLIA [Internet]. IEEE SigPort; 2018. Available from : http://sigport.org/3592